Table 2. Substrate specificities of NfBGL1.
Substrate | Specific activity(U/mg)a | Substrate | Specific activity (U/mg) |
pNPG | 2189±1.7 | Cellobiose | 203.8±0.9 |
p-Nitrophenyl β-d-cellobioside | 601.0±0.9 | Lichenin | 93.3±1.2 |
p-Nitrophenyl β-d-xylopyranoside | 6.6±0.3 | Daidzin | 90.1±0.7 |
p-Nitrophenyl β-d-galactoside | – | Glycitin | 75.2±0.6 |
p-Nitrophenyl α-l-arabinofuranoside | – | Genistin | 45.6±0.3 |
p-Nitrophenyl α-l-arabinopyranoside | – | Beechwood xylan | 6.4±0.5 |
Sophorose | 896.1±0.9 | Birchwood xylan | 6.2±0.7 |
Laminiarin | 675.4±1.1 | Lactose | 3.5±0.3 |
Gentiobiose | 602.4±0.8 | Xyloglucan | – |
Amygdalin | 510.8±0.7 | Avicel | – |
Barley β-glucan | 269.4±1.3 | CMC-Na | – |
Salicin | 246.6±0.6 |
Values are means ± S.D. (n = 3); –, no activity detected.