Table 3. Conversion of soybean isoflavone glycosides into free isoflavones by NfBGL1 and Novozyme 188. a .
| Enzyme | Isoflavone glycosides (mg/g) | Free isoflavonones (mg/g) | ||||
| Daidzin | Genistin | Glycitin | Daidzein | Genistein | Glycitein | |
| Control | 146.8±0.4 | 68.7±0.3 | 54.0±0.2 | 100.8±0.7 | 220.7±1.1 | 21.7±0.4 |
| NfBGL1 | 0 | 25.9±0.4 | 0 | 222.4±0.9 | 598.2±0.6 | 73.4±1.0 |
| Novozyme 188 | 0 | 32.7±0.6 | 16.9±0.1 | 253.6±0.8 | 746.5±1.0 | 70.3±1.1 |
a
The reaction system without enzyme addition was treated as the control; the data are shown as mean ± S.D. (n = 3).