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. 2014 Sep 4;9(9):e106703. doi: 10.1371/journal.pone.0106703

Figure 3. The effects of BMPR2 mutations on HLMVE cell apoptosis.

Figure 3

Imunofluorescent images of HLMVE cells transfected with BMPR2 wild-type (WT) or mutant plasmids exposed to serum-free medium for 24 hours. TUNEL-positive cell nuclei exhibit bright red fluorescence as opposed to the blue fluorescence of the DAPI nuclear counterstain, whereas co-localization of TUNEL and DAPI staining appears as pink. B, Summary data showing the proportion of TUNEL-positive nuclei in HLMVE cells transfected with BMPR2 wild-type or mutant plasmids. *P<0.05 compared with wild-type.