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. 2014 Sep 4;9(9):e107074. doi: 10.1371/journal.pone.0107074

Figure 3. Relative contribution of gp120 and Tat to neurotoxicity.

Figure 3

HIV-1-infected MDM supernatants (100 µl) from representative isolates US clade B (HIV-1B:HIV-1ADA), Southern African (HIV-1C:HIV-11084i) and Indian clade C HIV-1 (HIV-1C:HIV-1IndieC1) with equal p24 loads were diluted in SH-SY5Y media and added to SH-SY5Y cultures and incubated for 24 h. Media with and without immuno-depletion of gp120 and Tat were tested. Medium indicates untreated MDM supernatants, while ‘Medium minus gp120’ and ‘Medium minus gp120 and Tat’ refer to gp120- immunodepleted and gp120 and Tat-depleted media respectively. WST-1 assay was performed to determine neuronal death. (**p<0.01; ***p<0.005).