Skip to main content
. 2014 Sep 4;9(9):e106804. doi: 10.1371/journal.pone.0106804

Figure 7. The recombinant Lgr4-ED protein decreases gonadotropin-driven ovarian development and steroidogenesis.

Figure 7

Immature female rats (26 days old) were intraperitoneally injected with PMSG in the presence or absence of 20 µg Lgr4-ED. The ovaries were harvested two days later for assessing the expression levels of (A) Esr1, Esr2, Aqp1 and Lhr. β-actin was used for normalization. (all bars; n = 7). In addition, (B) the progesterone amounts and (C) the transcript levels of various steroidogenic genes in the ovaries were evaluated (n = 4). Data are shown as the mean ± SEM. Asterisks indicate P<0.05.