Fig. 4.

Inhibition of Akt kinase activity by perifosine in living mice. a The efficacy of perifosine in inhibition of Akt kinase activity in living mice was determined by bioluminescence imaging of nude mice bearing 293T/ASA (left) and 293T/AST (right) xenografts. Upon determination of base-line signals at time 0 h, mice were treated with 30 mg/kg of perifosine or carrier control and re-imaged at 6, 15, 27, and 39 h upon i.p. injection of D-luciferin. b Max photons at each time point was normalized to that of carrier control treated mice and expressed as average normalized max photons±SEM for each xenograft. *p<0.05 relative to mice bearing 293T/ASA xenografts.