Table 1.
Fluorescent, bioluminescent and PETa reporter gene expressions exhibited by different triple fusion constructs in comparison to the respective positive controls assayed from transiently transfected 293T cells (tTK and hRL activities are normalized with cotransfected β-Gal activity)
| Constructs | % TK activity | % wTK activity | % hRL activity | % FL activity | RFP/eGFP/mRFP (fluorescence activity by microscopy) |
|---|---|---|---|---|---|
| hrl-rfp-ttk | 39.7 | 27.8 | Medium | ||
| fl-rfp-ttk | 29.6 | 22.1 | Low | ||
| hrl-egfp-ttk | 50 | 33 | High | ||
| fl-egfp-ttk | 43 | 20 | High | ||
| hrl-mrfp-ttk | 149 | 54 | High | ||
| fl-mrfp-ttk | 100 | 53.6 | Medium | ||
| hrl-rfp-wttk | 76 | 44.7 | Medium | ||
| fl-rfp-wttk | 61.6 | 62.6 | Low | ||
| ttk | 100 | ||||
| wttk | 100 | ||||
| hrl | 100 | ||||
| fl | 100 | ||||
| mrfpl | Very high |
a
PET, positron emission tomography; eGFP, enhanced green fluorescence protein; β-Gal, β-galactosidase.