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. 2014 Sep 4;9(9):e105835. doi: 10.1371/journal.pone.0105835

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© 2014 Takano et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) RT-PCR results for GABA_B receptor subtypes in rat kidney cortex. Appropriately-sized bands were detected for GABA_B receptor R1 and R2 subtypes in at least five independent experiments. Rat brain RNA or commercial universal RNA was used as positive control. M: molecular marker. B) Immunostaining for GABA_B receptor R1 and R2 subtypes in rat kidney cortex. Staining with normal IgG instead of primary antibody served as negative control.