Table 1. Primer information.
Gene | Forward primer (3′–5′) | Reverse primer (3′–5′) | Accession No.or assay ID | Ampliconsize (bp) |
GABAA α1 | tgacagtcattctctcccaagtc | tcagaacggtcgtcactcc | NM_183326 | 87 |
GABAA α2 | gactgtcattctctcccaagtgt | tcattgtcaaaacagttgttactcc | NM_001135779 | 92 |
GABAA α3 | cctacttgccatgtatcatgactg | ggtcatggtgagaacagtggt | NM_017069 | 113 |
GABAA α4 | cttctggatcaataaggagtctgtt | tcatcgtgaggactgtggtt | NM_080587 | 68 |
GABAA α5 | gccttggaagcagctaaaatc | gtacccacagcattcccagt | NM_017295 | 146 |
GABAA α6 | agtcccagaaagccgaaag | ttcttcagatggtacttggagtca | NM_021841 | 124 |
GABAA β1 | ccctctggatgagcaaaact | ccctctcctccattccagta | NM_012956 | 90 |
GABAA β2 | catcgatatggtttctgaagtcaa | gggattacattgtaggacagtctctt | NM_012957 | 96 |
GABAA β3 | atcgagctcccacagttctc | tcaatgagagtcgagggtagg | NM_017065 | 91 |
GABAA γ1 | gatgcgcactggataacaac | tggagttgaaggtagcattctg | NM_080586 | 110 |
GABAA γ2 | acagaaaatgacgctgtgga | atctgacttttggctagtgaagc | NM_183327 | 70 |
GABAA γ3 | agcgagtggagaccaagc | ctcttccaccctcctggac | NM_024370 | 124 |
GABAA δ | aatgacattggggactacgtg | gccacattcactggaggac | NM_017289 | 125 |
GABAA ε | ccgctgagatgttgcctaa | tccagtttaatgtgaggtccaa | NM_023091 | 88 |
GABAA π | gttgcagctggaaagttgg | gagtccacgtaccgaatcgt | NM_031029 | 82 |
GABAA θ | tgatgtccgactgagaccaa | tctgagatctgttcaatactggagac | NM_031733 | 87 |
GAD65 | gtacgccatgctcattgccc | agagaggatcaaaagccccg | NM_012563 | 299 |
GAD67 | gctggaaggcatggaaggtttta | acgggtgcaatttcatatgtgaacata | NM_017007 | 222 |
GAT1 | ttcaagggtgtgggcctcgc | ccacggcagagtcgtggtga | NM_024371 | 120 |
GAT2 | gcagcgaacacaagcgcatcc | atcccacctgcagccgctact | NM_133623 | 126 |
GAT3 | tcgtgttgagcgtagcgggag | ggaatgcccctccgccgtt | NM_024372 | 90 |
ABAT | cctgggcatcctgcctccaga | ttgggcgccaccgacatca | NM_031003 | 70 |
GABAB R1 | Rn00578911_m1 | 113 | ||
GABAB R2 | Rn00582550_m1 | 87 | ||
GABAC ρ1 | Rn00568768_m1 | 61 | ||
GABAC ρ2 | Rn00568783_m1 | 86 | ||
GABAC ρ3 | Rn00594210_m1 | 88 |
Abbreviations: GABA; γ-aminobutyric acid, GAD; glutamate decarboxylase, GAT; GABA transporter, ABAT; 4-aminobutyrate aminotransferase.