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. 2014 Sep 4;9(9):e105732. doi: 10.1371/journal.pone.0105732

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© 2014 Kern et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Mixed asexual blood stage cultures containing trophozoites (TZ) and schizonts (SZ) and mature gametocyte (GC) cultures were fixed with methanol and prepared for IFA, using rat antisera against PfCLK-3 and mouse antisera against PfCLK-4 (green). The parasite nuclei were highlighted by Hoechst staining (blue). The asexual blood stages were labelled with rabbit antisera against PfMSP-1 and gametocytes with rabbit antisera against Pfs230 (red). Bar, 5 µm.