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. 2014 Sep 4;9(9):e106085. doi: 10.1371/journal.pone.0106085

Figure 2. Virulent S. flexneri serotype 3b accumulates in C. elegans intestinal lumen 24 hours post infection.

Figure 2

A: Young adult hermaphrodite N2 nematodes were fed either E. coli OP50, avirulent S. flexneri (SFL1223) or virulent S. flexneri 3b (SFL1520) for 24 hours at 22°C. 20 worms were picked off each bacterial lawn, disrupted using glass beads and appropriate dilutions of each lysate were plated on LB agar to obtain bacterial counts. Wild type S. flexneri 3b (SFL1520) accumulated in the intestinal lumen while the plasmid-cured S. flexneri strain (SFL1223) and OP50 were digested (p<0.001, One way ANOVA). Results represent the means of three independent experimental repeats with standard errors (error bars). B: Young adult hermaphrodite N2 nematodes were fed on lawns of E. coli OP50, avirulent S. flexneri (SFL1223) or virulent S. flexneri 3b (SFL1520) tagged with GFP+ for 24 h and fluorescence was observed using an EVOS digital inverted microscope (AMG). Scale bar = 400 µm.