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. 2014 Sep 4;9(9):e106085. doi: 10.1371/journal.pone.0106085

Figure 6. Effects of knockdown of S. flexneri-induced host response genes on nematode survival.

Figure 6

After RNAi knockdown of S. flexneri-induced genes aco-1 (B), cct-2 (C), daf-19 (D) and hsp-60 (E), young adult hermaphrodites were transferred onto lawns of either E. coli OP50, avirulent S. flexneri (SFL1223) or virulent S. flexneri serotype 3b (SFL1520) and scored at 12 hour intervals for survival. A: Nematodes treated with pCB19 (RNAi control vector) were used as a non-specific ds-RNA control. Survival curve represents data from three independent experiments, each using 20 nematodes. The survival curves of RNAi worms infected with S. flexneri serotype 3b were compared with RNAi control-pCB19 worms infected with S. flexneri serotype 3b (grey, dashed curves); and asterisks indicate statistically significant difference in survival curves (p<0.05) identified using Log rank tests.