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. 2014 Sep 4;9(9):e106085. doi: 10.1371/journal.pone.0106085

Table 1. Predicted S. flexneri-induced C. elegans responses using DIGE analysis.

Identifiedprotein Genename Shigella-inducedchange inexpression Description PredictedpI/Mwt (kDa) ObservedpI/Mwt (kDa) PeptideCoverage(%) No ofpeptidesMatched Score P value(One WayANOVA)
Putative stonedB-like protein unc-41 Down-regulated Potential adapter protein,which may be involved inendocytic vesicle recyclingof synaptic vesicles 4.81/190.58 4.8/∼94.00 3 7 27 0.029
Myosin-4 unc-54 Up-regulated Encodes a muscle myosinclass II heavy chain (MHC B).Expressed in the intestine.Involved in pharyngealpumping and egg laying 5.59/225.958 5.2/96.00 8 5 134 0.038
Probable cytoplasmicaconitate hydratase aco-1 Up-regulated Enzyme that catalyzes theisomerization of citrate toisocitrate via cis-aconitate.Required for iron homeostasis 5.49/97.11 5.5/95.00 14 5 86 0.022
Elongation factor 2 eef-2 Up-regulated Catalyzes the GTP-dependentribosomal translocation stepduring translation elongation.Required for embryogenesis 6.1/95.47 5.5/95.00 18 8 72 0.022
RFX-liketranscription factor daf-19 Up-regulated A transcription factor thatregulates genes of ciliatedsensory neurons. daf-19mutants are defective in theirability to taste or smell and aresusceptible to bacterial infection! 5.97/91.42 5.1/86.00 2 3 24 0.040
Chaperonin homologHsp-60 hsp-60 Up-regulated Heat shock protein implicated inmitochondrial proteinimport and macromolecularassembly. Required forresponse to oxidative stress 5.31/60.24 5.1/68.00 49 20 1446 0.040
T-complex protein1 subunit beta cct-2 Up-regulated Molecular chaperone; assiststhe folding of proteinsupon ATP hydrolysis. 5.65/53.38 5.6/67.5 6 2 41 0.047