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. 2014 Apr 17;10(2):177–185. doi: 10.4161/org.28819

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Copyright © 2014 Landes Bioscience

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graphic file with name org-10-177-g4.jpg — Figure 4. Hedgehog signaling is perturbed in the talpid³ liver during development. IHC anti-acetylated (cilia axonemes, green) and γtubulin (centrosomes, red) E6 (A andB). Wt liver has cilia axonemes projecting from centrosomes (circled) (A) cilia axonemes are not observed projecting from centrosomes in the talpid³ liver (circled) (B). (C) Real-time PCR identified a 0.08-fold reduction in PTCH1 expression in the day 6 talpid³ liver, compared with the wt.