Figure 2. Pig liver scaffold preparation. Porcine livers can also be harvested and efficiently decellularized with an identical technique as in human cadaveric donor retrieval, using cold perfusion with preserving solution via portal vein and hepatic artery. After back-table preparation, these organs were connected to a pressure controlled perfusion system, continuously infusing a decellularization solution for 24 h based on SDS. This particular system included a remote controlled pump with a pressure sensor all connected to a computer hosting the controlling software (Velasco et al. unpublished data).