Table 1. Studies in the literature.
| Author | Type Cells | Infusion Method | Via | Number Cells | Flow Rate | Time |
|---|---|---|---|---|---|---|
| Baptista 2010 |
hUVEC hFLC MS1 |
Continuous | PV IVC, PV, IVC+PV |
30x106 70 x106 100 x106 |
3ml/min → 0.5ml/min 5ml/min |
7d 3d |
| Uygun 2010 |
Rat MH Endothelial cells |
Multistep | PV | 200 x106 | 15ml/min | 5d 5d |
| Soto 2011 |
Rat MH | Direct injection Continuous Multistep |
PV | 10–50 x106 | 2ml/min | 7d |
| Yagi 2013 |
Pig MH | Multistep | PV | 100 x106 | 4ml/min | 7d |
Methods adopted by several authors in liver bioengineering. From endothelial or human fetal liver cells to mature hepatocytes, several authors have been able to re-cellularize with success liver acellular scaffolds using different methods. Abbreviations: hUVEC, human umbilical vein endothelial cell; PV, portal vein; hFLC, human fetal liver cell; IVC, inferior vena cava; MH, mature hepatocyte.