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(A) Experimental (wide bars) and recolored (narrow bars) FRET efficiency histograms of the Alexa 488/Alexa 647-labeled WW domain immobilized on the glass surface at various solvent viscosities. The FRET efficiency histograms (1 ms bin time) were constructed from the trajectories with the mean photon count rate > 50 ms-1. Figures from Ref. 36. (B) Parameters obtained by maximizing the likelihood functions in Eq. (1) (circles) and Eq. (4) (green crosses and squares).
