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. Author manuscript; available in PMC: 2015 Sep 21.
Published in final edited form as: Phys Chem Chem Phys. 2014 Sep 21;16(35):18644–18657. doi: 10.1039/c4cp02489c

Figure 6.

Figure 6

The dependence of the maximum likelihood parameters on the ratio of the photon count rates in the folded and unfolded states (nF / nU). The amount of the simulated data is the same as that in Fig. 5 and k/n = 0.02 (square) and 0.2 (circle) and pF = 0.5. nF and nU were varied so that the average count rate (nF + nU)/2 is equal to 50 ms-1. The data were analyzed using the likelihood functions with FRET efficiencies (Eq. (1), blue) and with photon count rates (Eq. (4), orange). The error bars indicate the standard deviation obtained from 5 simulation data set. In the plots of E and k/n, all errors are smaller than the size of markers.