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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Bone Marrow Transplant. 2014 Jul 21;49(9):1198–1204. doi: 10.1038/bmt.2014.137

Figure 6. Apoptotic effect of extracorporeal photopheresis (ECP) on marrow transplantation.

Figure 6

Whole blood and buffy coat cells obtained before and after photopheresis were cultured overnight at 37°C, 5% humidified atmosphere, lyzed, washed, stained with Annexin-V-FITC, and propodium iodide (Pi), and analyzed with FACScan. (A) The mean ± SEM percentage of lymphocytes demonstrating early apoptosis (Annexin-V+/PI). (B) The mean ± SEM percentage of lymphocytes demonstrating late apoptosis (Annexin-V+/PI+)