TABLE 1.
DLA-nonidentical marrow transplantation after ECP ± pentostatin and 920cGy with postgrafting MTX†
| Recipient Dog # | Buffy Coat TNCs × 108/kg | Marrow TNCs × 108/kg | Neutrophil Increasec | % Donor MNC Chimerism (Max-final) | Rejection | GVHDd | Survival (days) | Cause of Death | |
|---|---|---|---|---|---|---|---|---|---|
| Group A1a | E981 | 4.3 | 1.9 | Yes | 100-100 | No | S/G/L | 17 | GVHDe |
| G120* | 3.9 | 2.2 | Yes | 36 | No | S/G/L | 16 | GVHDe | |
| G113 | 6.9 | 3.4 | Yes | 100-0 | Yes | L | 50 | End of study | |
| G066 | 6.8 | 6.1 | Yes | 100-100 | No | S/G/L | 19 | GVHDe | |
| G170 | 2.3 | 2.8 | No | 67–100 | No | G/L | 20 | GVHDe | |
| Group A2b | G077 | 2.1 | 3 | No | 41–73 | No | N | 9 | Herpes infectione |
| G088 | 2.2 | 3.1 | Yes | 95–100 | No | S/G/L | 22 | GVHDe | |
| G095 | 1.8 | 2.7 | No | 71-0 | Yes | N | 22 | End of Study | |
| G227 | 1.2 | 2.9 | No | 74 | No | S/G/L | 10 | GVHDe |
Dogs given marrow grafts and additional donor PBMC from DLA-nonidentical littermates after conditioning with 920 cGy TBI and post grafting immunosuppression with methotrexate
DLA-nonidentical dogs given marrow grafts, extracorporeal photopheresis (ECP) product, pentostatin, and donor buffy coat cells after conditioning with 920 cGy total body irradiation (TBI) and postgrafting immunosuppression with methotrexate (MTX).
Dogs in group A1 received ECP product on days −2 and −1; 920 cGy TBI on day 0; donor buffy coat cells on days 1 and 2; MTX (0.4 mg/kg i.v. on days 1,3, 6, 11, and then weekly thereafter until day 102.
Dogs in group A2 received ECP product on days −6 and −5; 4 mg/m2 pentostatin i.v. on days −4, and −3; 920 cGy TBI on day 0; buffy coat and MTX regimen as in group A1.
Absolute neutrophil count increase greater than 500/μL.
S=skin; G=gut; L=liver.
Dogs were euthanized because of poor condition.
No DNA sample was obtained on day 7 after HCT.