Figure 3. NACK is a transcriptional target of Notch signaling.

A. mRNA expression in mouse lymphoma cells expressing exogenous N1^ICD or wt mouse thymocytes. B. mRNA expression in MEFs infected with N1^ICD. C. NACK expression in HC11 cells infected with N1^ICD, N2^ICD, N3^ICD, or N4^ICD. Histogram shows mean (SEM). D. Schematic representation showing architecture of the NACK promoter. Numbers denote genomic sequence from − to + with respect to the translation initiation codon ATG. TSS, transcription start site. E. ChIP of Notch on the NACK promoter in OE33 cells.