miR-409 inhibits tumor suppressor genes in prostate cancer. A, mRNA targets of miR-409-5p: STAG2, RBL2, RSU1 and NPRL2 and mRNA targets of miR-409-3p: RSU1, PHC3 and TUSC1, assayed by triplicate wells in qRT-PCR of ARCaPE and ARCaPM cells. The representative RT-PCR is shown. The experiment was repeated twice. B, Western analysis of STAG2 and RSU1 in ARCaPE and ARCaPM PCa cells. C and D, Cytoscape images of miR-409-3p and miR-409-5p signaling pathways. *: p<0.05 were considered to be statistically significant by t-test.