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. 2014 Aug 26;15:100. doi: 10.1186/1471-2202-15-100

Figure 1.

Figure 1

GH and GHR expression in SGZ-derived neurospheres. A) Evolution of neurosphere size after 7 (left), 12 (middle) or 15 (right) days in culture. B) Evolution of neurosphere number after 7-9 (left) or 13-15 (right) days in culture. In both cases, neurospheres were cultured in DMEM containing 1% Gibco B27 supplement, EGF (10 ng/mL) and FGF2 (10 ng/mL). C to E) SGZ-derived neurospheres express GHR and GH. Neurospheres were cultured for 7 days in DMEM containing 1% Gibco B27 supplement, and GH, GHR, GFAP and nestin immunoreactivities were detected with specific antibodies. GHR immunoreactivity is shown in red (C, D). GH immunoreactivity is shown in red (E). GFAP immunoreactivity is shown in green (C, E). Nestin immunoreactivity is shown in green (D). Scale bar = 10 μm.