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. 2014 Aug 26;15:100. doi: 10.1186/1471-2202-15-100

Figure 3.

Figure 3

GH treatment prevents apoptosis in SGZ neurospheres. A) Neurospheres growing in defined media were placed in defined media without EGF and FGF2, and 24 h later treated with GH (500 ng/mL), pegvisomant (20 μg/mL), or GH + pegvisomant for an additional 48 h period. Control cells were treated with saline. Cell apoptosis was determined by TUNEL staining. Each bar represents the mean + SEM of 3 experiments in triplicate. * = p <0.05 vs Control; ° = p <0.05 vs Control and p <0.01 vs GH. Basal apoptosis was determined by growing the cells in the defined media (Control + EGF + FGF2). Apoptosis values in this group were significantly lower (p <0.01) than in the other groups. E = EGF; F = FGF2. B) Representative images of results presented in A. Scale bar = 10 μm.