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. 2014 Aug 21;12:268. doi: 10.1186/1477-7819-12-268

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© Hwang et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Sonic hedgehog signaling and non-small-cell lung cancer cell proliferation. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide viability assays were performed to measure cell proliferation. (A) Proliferation of H1299 and H2009 cells treated with cyclopamine-KAAD (10 μM). (B) and (C) Proliferation of H1299 and Calu-1 cells transfected with Gli1, Gli2 or Gli3 siRNA or nonspecific control (Con) siRNA. Bars represent the standard deviations of three independent experiments conducted in triplicate. (D) H1299 and Calu-1 cells were transfected with Gli1, Gli2 or Gli3 siRNA or nonspecific control siRNA and harvested 48 hours later. RT-PCR was performed for endogenous Gli1, Gli2 and Gli3 transcripts. β-actin was used as a loading control.