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2D-PAGE analysis of PBMC extracts. Protein extracts (60 µg) from normal (A) and STZ-induced diabetic PBMC (B) were separated by IEF on Immobiline™ DryStirp pH 3–10 NL and then by SDS-PAGE. The gel was stained with silver staining. Rectangle represents the protein spot analyzed by MALDI-TOF mass spectrometry.
