Table 1. Sequences of the oligonucleotide primers used for quantitative real-time PCR.
Primer set | Primer sequence (5′-3′) | Position of the primers | GenBank accession number | |
---|---|---|---|---|
PAR-2 | Forward | TGA AGA TCG CCT ACC ACA TCC A | 389–525 | AB458680 |
Reverse | CCA ATA CCG TTG CAC ACT GA | |||
IL-1β | Forward | ACC CGA ACT CAC CAG TGA AAT G | 12–121 | NM_001037971 |
Reverse | GGT TCA GGT CTT GGC AGC AG | |||
TNF-α | Forward | CCC AAG TGA CAA GCC AGT AGC TC | 249–394 | NM_001003244 |
Reverse | ACA ACC CAT CTG ACG GCA CTA TC | |||
MCP-1 | Forward | CAC CTG CTG CTA TAC ACT CAC C | 97–196 | NM_001003297 |
Reverse | GAT CAC AGC TTC TTT GGG ACA | |||
MIP-3α | Forward | ATC ATG GGC TTC ACA CAA CA | 139–256 | NM_001005254 |
Reverse | TCC GTT TCA CCC ATT TCT TC | |||
IL-8 | Forward | CTT CCA AGC TGG CTG TTG CTC | 11–183 | NM_001003200 |
Reverse | TGG GCC ACT GTC AAT CAC TCT C | |||
TECK | Forward | GCT GCT TAG CCT ACC ACC AC | 86–228 | NM_001005259 |
Reverse | TGG GTT CAC ACA CAG CAT CT | |||
MEC | Forward | CAG ACA GGA CTC ACT CTC GCT CTC | 7–113 | NM_001005257 |
Reverse | TGT GAA ACC TCA GTG CAA CAG CTA | |||
Fractalkine | Forward | CTT CCT TGG CCT CCT CTT CT | 1119–1265 | AB648939 |
Reverse | GGC ACC AGG ACA TAC GAG TT | |||
TBP | Forward | CTA TTT CTT GGT GTG CAT GAG G | 72–167 | XM_849432 |
Reverse | CCT CGG CAT TCA GTC TTT TC | |||
GAPDH | Forward | CAT TGC CCT CAA TGA CCA CT | 894–998 | NM_001003142 |
Reverse | TCC TTG GAG GCC ATG TAG AC | |||
SDHA | Forward | GCC TTG GAT CTC TTG ATG GA | 855–946 | XM535807 |
Reverse | TTC TTG GCT CTT ATG CGA TG |
PAR-2: Protease-activated receptor-2, IL-1β: Interleukine-1 beta, TNF-α: Tumor necrosis factor alpha, MCP-1: Monocyte chemotactic protein-1, MIP-3α: Macrophage inflammatory protein-3 α, TECK: Thymus-expressed chemokine, MEC: Mucosae-associated epithelial chemokine, TBP: TATA box binding protein, GAPDH: Glyceraldehyde-3-phosphate dehydrogenase, SDHA: Succinate dehydrogenase complex subunit A.