Skip to main content
. 2014 May 15;76(8):1119–1127. doi: 10.1292/jvms.14-0060

Table 1. Sequences of the oligonucleotide primers used for quantitative real-time PCR.

Primer set Primer sequence (5′-3′) Position of the primers GenBank accession number
PAR-2 Forward TGA AGA TCG CCT ACC ACA TCC A 389–525 AB458680
Reverse CCA ATA CCG TTG CAC ACT GA
IL-1β Forward ACC CGA ACT CAC CAG TGA AAT G 12–121 NM_001037971
Reverse GGT TCA GGT CTT GGC AGC AG
TNF-α Forward CCC AAG TGA CAA GCC AGT AGC TC 249–394 NM_001003244
Reverse ACA ACC CAT CTG ACG GCA CTA TC
MCP-1 Forward CAC CTG CTG CTA TAC ACT CAC C 97–196 NM_001003297
Reverse GAT CAC AGC TTC TTT GGG ACA
MIP-3α Forward ATC ATG GGC TTC ACA CAA CA 139–256 NM_001005254
Reverse TCC GTT TCA CCC ATT TCT TC
IL-8 Forward CTT CCA AGC TGG CTG TTG CTC 11–183 NM_001003200
Reverse TGG GCC ACT GTC AAT CAC TCT C
TECK Forward GCT GCT TAG CCT ACC ACC AC 86–228 NM_001005259
Reverse TGG GTT CAC ACA CAG CAT CT
MEC Forward CAG ACA GGA CTC ACT CTC GCT CTC 7–113 NM_001005257
Reverse TGT GAA ACC TCA GTG CAA CAG CTA
Fractalkine Forward CTT CCT TGG CCT CCT CTT CT 1119–1265 AB648939
Reverse GGC ACC AGG ACA TAC GAG TT
TBP Forward CTA TTT CTT GGT GTG CAT GAG G 72–167 XM_849432
Reverse CCT CGG CAT TCA GTC TTT TC
GAPDH Forward CAT TGC CCT CAA TGA CCA CT 894–998 NM_001003142
Reverse TCC TTG GAG GCC ATG TAG AC
SDHA Forward GCC TTG GAT CTC TTG ATG GA 855–946 XM535807
Reverse TTC TTG GCT CTT ATG CGA TG

PAR-2: Protease-activated receptor-2, IL-1β: Interleukine-1 beta, TNF-α: Tumor necrosis factor alpha, MCP-1: Monocyte chemotactic protein-1, MIP-3α: Macrophage inflammatory protein-3 α, TECK: Thymus-expressed chemokine, MEC: Mucosae-associated epithelial chemokine, TBP: TATA box binding protein, GAPDH: Glyceraldehyde-3-phosphate dehydrogenase, SDHA: Succinate dehydrogenase complex subunit A.