. Author manuscript; available in PMC: 2015 Mar 3.

Published in final edited form as: Nat Commun. 2014 Sep 3;5:4794. doi: 10.1038/ncomms5794

Table 1.

The effect of the identified PB1 mutations on ribavirin sensitivity and polymerase activity using mini-genome assays.

PB1 Mutation	Ribavirin sensitivity at the polymerase level (EC₅₀, μM)	Polymerase enzyme activity (Fold change)^*
Wild-type	41.67 (36.24 to 47.91)	1.00
V43I	95.59 (70.92 to 128.8)	0.50
V191I	60.21 (57.23 to 63.35)	0.91
A661T	68.32 (63.68 to 73.31)	1.00
S741F	68.64 (64.04 to 73.56)	0.42
RNP^rib-resist	78.68 (70.20 to 88.19)	0.71

Fold-change relative to wild-type

Fold change in polymerase activity was determined by dividing the polymerase activity (firefly per renilla luciferase relative luminescence) by that of wild-type RdRP. Mean EC₅₀ in 95% confidence level (in parentheses) values from 3 replicates are shown. The experiment was repeated twice and one representative result is shown.