Table 1.
The effect of the identified PB1 mutations on ribavirin sensitivity and polymerase activity using mini-genome assays.
| PB1 Mutation | Ribavirin sensitivity at the polymerase level (EC50, μM) | Polymerase enzyme activity (Fold change)* |
|---|---|---|
| Wild-type | 41.67 (36.24 to 47.91) | 1.00 |
| V43I | 95.59 (70.92 to 128.8) | 0.50 |
| V191I | 60.21 (57.23 to 63.35) | 0.91 |
| A661T | 68.32 (63.68 to 73.31) | 1.00 |
| S741F | 68.64 (64.04 to 73.56) | 0.42 |
| RNPrib-resist | 78.68 (70.20 to 88.19) | 0.71 |
*
Fold-change relative to wild-type
Fold change in polymerase activity was determined by dividing the polymerase activity (firefly per renilla luciferase relative luminescence) by that of wild-type RdRP. Mean EC50 in 95% confidence level (in parentheses) values from 3 replicates are shown. The experiment was repeated twice and one representative result is shown.