FIG. 7.

Comparative expression analysis of osteogenesis-related genes between iPSCs and MSCs. Expression of 84 osteogenesis-related genes (listed in the Supplementary Table S1) on days 10 (A) and 30 (B) of osteogenic induction was evaluated using real-time RT-PCR array analysis (Mouse Osteogenesis RT² Profiler PCR Array: Qiagen). Expression levels (2^−ΔCT) of these genes are plotted on a logarithmic scale. The two boundary lines above and below the center partition line indicate the threshold of 10-fold up-regulation and down-regulation in iPSCs relative to MSCs. Genes with at least 10-fold higher (in gray) or lower (in italics) expression in iPSCs compared with MSCs are shown as black dots (the gene names for the abbreviations are given in Supplementary Table S1). The Pearson correlation coefficients (r) of the spotted genes are indicated.