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. 2014 Jan 11;23(18):2180–2189. doi: 10.1089/scd.2013.0424

FIG. 2.

FIG. 2.

Early growth response protein-1 (EGR-1) increases in the hES cells treated with PMA. (A) DNA microarray analysis of H9 hES cells treated with or without PMA for 1 h. (B) DNA microarray analysis of H9 hES cells treated with PMA or PMA together with GFX for 1 h. (C) Venn diagram of probes (purple) shared by the PMA-influenced probes (red) and GFX-influenced probes (blue) in DNA microarray analysis. Values in Venn diagram indicated the number of probes changed (fold change ≥5.0) by DNA microarray analysis at (A) and (B). (D) The fluorescence intensity of each spot of EGR genes in the microarray was normalized against the fluorescence intensity of GAPDH gene. The data are represented as mean±standard error (SE, n=3). *P<0.05. (E) Immunostaining for EGR-1 (Alexa fluor 647; red) in H9 hES cells treated with PMA (10 nM) or PMA together with GFX (5 μM). Nuclei were stained with Hoechst 33342 (blue). Scale bars=50 μm.