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. 2014 Jan 11;23(18):2180–2189. doi: 10.1089/scd.2013.0424

FIG. 4.

FIG. 4.

EMT-related gene expression by ectopic EGR-1. (A) Western blot analysis of ectopic EGR-1 expression in the cells transfected with EGR1-2A-eGFP or with enhanced green fluorescent protein (eGFP) control plasmid. Transiently ectopic EGR-1 expression was confirmed by the specific EGR-1 antibody at 24 h after transfection. (B) qRT-PCR analysis of SNAIL in the cells expressing EGR1-2A-eGFP or eGFP alone in the presence or absence of GFX. Expression levels of the SNAIL gene were all normalized against GAPDH. The data are represented as mean±SE (n=3). *P<0.05. (C) Phase-contrast and fluorescence images of H9 hES cells transfected with pEGR1-2A-eGFP or peGFP control plasmid. The EGR1-2A-eGFP or eGFP alone expressing cells were selected on hygromycin B (200 μg/mL) in the hESF9 medium for 6 days. Scale bars=200 μm. (D) qRT-PCR analysis of the EMT-related genes. Expression levels of genes in the cells expressing EGR1-2A-eGFP (black bars) or eGFP alone (white bars) were all normalized against GAPDH. The data are represented as mean±SE (n=3). *P<0.05.