FIG. 1.

Generation of partial induced pluripotent stem cell (iPSC) clones. (A) Bright field and fluorescence images of partial iPSC clone 2B1 (upper panels) and those subjected to the 2i condition with MAPK and GSK3β inhibitors (lower panels). (B) Reverse transcription-PCR analyses of the expression of endogenous (end) and exogenous (exo) reprogramming factor genes and other indicated embryonic stem cell (ESC) marker genes in partial iPSCs (2B1) and those exposed to the 2i condition for 8 days. For the latter cells, GFP-positive cells were sorted and then expanded before the analyses. (C) Bisulfite sequencing analyses of pluripotency marker genes in partial iPSC clone 2B1. The analyses were also conducted with mouse embryonic fibroblasts (MEFs) and ESCs as references. (D) Hierarchical clustering of whole-genome transcripts in partial iPSC clones 2B1 and 5C5, and iPSCs generated from them under the 2i condition together with those from different laboratories [6,7]. Partial iPSC clones 2B1 and 5C5 and their derivative iPSCs are indicated with red and blue letters, respectively. Tree branch heights are proportional to the distances between samples.