FIG. 3.

Effect of knockdown of Cnot1, Cnot2, Cnot3, or Trim28 in partial iPSCs on the production of Nanog-GFP-positive iPSCs. Partial iPSCs (2B1) were infected with a retrovirus expressing a puromycin-resistance gene and shRNA targeting the indicated genes. Transfectants were enriched by puromycin selection (1.5 μg/mL) and then exposed to the 2i condition for iPSC conversion. GFP-positive cells were quantified by flow cytometry. Data are the mean±standard deviation (n=3). **P<0.01. Lower panels show western blots examining the efficiency of knockdown.