Table 2.
Techniques Applied in GEAR.
| Moore summer 2012 class | Moore summer 2013 class | Teter fall 2012 class |
|---|---|---|
| Lab safety | Lab safety | Lab safety |
| Aseptic technique | Aseptic technique | Aseptic technique |
| Pipet-Aid / pipetman use | Pipet-Aid / pipetman use | Pipet-Aid / pipetman use |
| Lab math | Lab math | Lab math |
| Lab documentation | Lab documentation | Lab documentation |
| Use of balances and pH meter | Use of balances and pH meter | Use of balances and pH meter |
| Plasmid preparations | Agarose gel electrophoresis | Plasmid preparations |
| Bacterial plating and culturing | Conventional PCR | Spectrophotometry |
| Recombineering | Use of Excel for modeling PCR | Transformation by electroporation |
| Spectrophotometry | Quantitative, real-time PCR | SDS-PAGE |
| Transformation by electroporation | cDNA preparation from RNA | Western blot |
| Agarose gel electrophoresis | Dialysis | |
| Preparation of electrocompetent E. coli | Bradford assay | |
| Transduction | Talon affinity chromatography | |
| PCR | Pull-down assay | |
| Co-immunoprecipitation |