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. Author manuscript; available in PMC: 2015 Mar 3.
Published in final edited form as: Nat Commun. 2014 Sep 3;5:4787. doi: 10.1038/ncomms5787

Figure 2. Migration patterns of human neutrophils in response to fMLP, LTB4, C5a and IL-8.

Figure 2

A) Percentage of human neutrophils loaded in the cell-loading channel, which migrate towards or away from fMLP, LTB4, C5a and IL-8 at different concentrations. Human neutrophils migrate in significantly greater numbers towards fMLP and LTB4 than away (p<0.001 and p<0.01 respectively, Student's t test). Human neutrophils migrate towards and away from C5a and IL-8 in similar proportions. Directionality and speed of neutrophils in response to B) fMLP (100nM, N=4), C) LTB4 (100nM, N=3), D) C5a (10 nM, N=4), E) IL-8 (120 nM, N=4), and F) cell culture media alone (N=6). Bars represent mean ± SD. For all graphs red color represents migration towards a chemokine and blue represents migration away from a chemokine.