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. Author manuscript; available in PMC: 2015 Mar 3.
Published in final edited form as: Nat Commun. 2014 Sep 3;5:4787. doi: 10.1038/ncomms5787

Figure 4. Migration of human T lymphocytes in response to SDF-1.

Figure 4

A). Percentage of human T lymphocytes migrating towards or away from SDF-1 (10 μg mL−1) and media (control) Significantly greater numbers of T cells (both pooled CD3+ and CD4+ cells) migrate away from SDF-1 than towards (p<0.05, Student's t test). B). Distribution of speed and bi-directional index of human CD3+ T lymphocytes in response to SDF-1 (10 μg mL−1). Average persistence of the cells migrating towards and away from SDF-1 are 0.15 and 0.27, respectively. Only ~1.2% of the lymphocytes migrated persistently towards SDF-1, while ~4.7 % of the lymphocytes in the device migrated away from SDF-1 with high persistence. C) Distribution of Bi-Directional Index of T-cell subpopulations. Persistence is very low in all subgroups. D) Histogram of speed of human CD3+ T lymphocytes in response to 10 μg mL−1 SDF-1 in both directions (total 564 cells analyzed: 368 repelled cells and 196 attracted cells). Bars represent mean ± SD. Graphs represent combined data of N=3 independent experiments.