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. 2014 Aug 4;35(9):1157–1166. doi: 10.1038/aps.2014.54

Figure 10.

Figure 10

Effect of AG490 on Ang II-induced STAT3 activation and expression of pro-fibrotic gene in NRK-52E cells. (A) Cells were treated with Ang II (1 μmol/L) for 1-h with or without a 1 h pretreatment of AG490 (12.5 μmol/L). Acetyl-STAT3 (Lys685), phospho-STAT3 (Tyr705) and phospho-STAT3 (Ser727) were detected by Western blot analysis. Data are presented as the mean±SEM of 3 experiments. cP<0.01, compared with control [Ang II (−) and AG490 (−)]. fP<0.01 compared with Ang II only. (B) Cells were treated with Ang II (1 μmol/L) for 48 h with or without a 1-h pretreatment of AG490 (12.5 μmol/L). Fibronectin was analyzed by Western blot analysis. Media containing Ang II or AG490 were changed every 24 h. Data are presented as the mean±SEM of 3 experiments. cP<0.01, compared with control [Ang II (−) and AG490 (−)]. fP<0.01 compared with Ang II only.