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. 2014 Aug 25;35(9):1199–1206. doi: 10.1038/aps.2014.60

Figure 1.

Figure 1

Mig-2 regulated cisplatin-induced apoptosis in glioma cells. (A) Endogenous expression of mig-2 in glioma cell lines HS 683, H4, and U-87 MG. (B) Ectopic expression or knock-down of mig-2 in glioma cell lines HS 683, H4, and U-87 MG were analyzed using Western blotting using an anti-mig-2 antibody. (C) Cells were stained using Annexin V/PI for analyzing cell apoptosis. Ectopic expression or knock-down of mig-2 in glioma cell lines. HS 683, H4, and U-87 MG cells were not treated or treated with cisplatin for 24 h. Then, the cells were stained with Annexin V/PI and analyzed by flow cytometry. The bar chart shows the percentage of apoptotic cells. (D) Effects of mig-2 on the viability of glioma cell lines HS 683, H4, and U-87 MG after treatment with cisplatin or a control reagent by MTS assay. The data are presented as the mean±SD from three independent experiments. bP<0.05 versus control.