Figure 4.

F3 subdomain of mig-2 is necessary and sufficient for mig-2 antagonizing cisplatin-induced apoptosis. (A) The ectopic expression of mutant and wild-type mig-2 in the glioma cells line H4 was confirmed by Western blotting using an anti-GFP antibody (arrows). (B) Analysis of cisplatin-induced apoptosis in mutants or wild-type mig-2 cells using Annexin V/PI staining. The bar chart shows the percentage of apoptotic cells (early apoptotic+late apoptotic). (C) Western blotting analysis of cleaved-PARP, cleaved-caspase-8, cleaved-caspase-9, cleaved-caspase-3, activation of Bid, Bax, Bcl-2, and cytochrome c. The total expression and activation of JNK, p38, and AKT after cells were treated or not treated with cisplatin for 24 h in mutant or wild-type mig-2 cells. The data are presented as the mean±SD from three independent experiments. ^bP<0.05 versus control.