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. Author manuscript; available in PMC: 2014 Sep 5.
Published in final edited form as: Diabetologia. 2013 Dec 4;57(3):603–613. doi: 10.1007/s00125-013-3128-1

Figure 1. Protein expression of MMP9 in the skeletal muscle and plasma MMP9 activity.

Figure 1

Gastrocnemius muscle was freeze-clamped following the ICv and was measured for the protein expression of MMP9 by Western Blotting. GAPDH was used as an internal control. (a) Representative bands. (b-c) Quantitative data. Data were normalized to GAPDH expression and presented as fold of chow-fed mmp9+/+. White bars for mmp9+/+ and black bars for mmp9−/−. (d) Gelatin zymography of plasma in mmp9+/+ mice after chow feeding (white bars) or HF feeding (black bars) for 20 weeks. N is equal to 3–6 for the female mice and 5–6 for the male mice. *p<0.05 when compared to mmp9+/+ mice with the same diet; p<0.05 when compared to chow-fed mice with the same genotype; p<0.05 when compared to chow-fed mice with the same gender.