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Normalization procedure for array data as shown on Atlas Clontech membranes. A. Histogram of averaged expression data from duplicated spots. B. Normality plot of A. C. Histogram of the trimmed data +/- 2SD about the mean. D. Histogram of the data after z transformation. E. Scatter plot showing the regression line for duplicated expressions (log transformed) after normalization. F. Scatter plot of background genes exhibiting the normality of their profile with average of 0 and SD of 1.
