TABLE 1.
SAXS data
The following abbreviation are used: I(0), interpolated intensity at 0 angle; Rg, radius of gyration; Vc, volume of correlation; Mp, molecular weight of protein; V, volume; Dmax, maximum pairwise distance; P(r), pair-distance distribution function; Rc, cross-sectional radius of gyration; Px, Porod exponent. A noticeably larger Rg, Dmax, and a translated P(r) peak in CAR(tcp):RXR(9c)·SRC suggest a significantly larger species than CAR(tcp):RXR·SRC. Close agreement of real and Guinier-derived values confirm that the data is internally consistent. We use the convention of 4π × sinθ/λ. χ 2 values are for DAMMIN envelope generation.
Molecular species | I(0) | I(0)_R | Rg | Rg_R | Vc | Mp | V | Dmax | P(r) peak | Rc | Px | χ2 |
---|---|---|---|---|---|---|---|---|---|---|---|---|
CAR(tcp):RXR(9c) | 0.05 | 0.046 | 35.20 | 32.01 | N/A | N/A | 1.43E + 005 | 97 | 32.25 | 17.88 | 3.8 | 0.755 |
SRC1 | 0.20 | 0.170 | 33.96 | 31.63 | 364 | 31.5 | 1.06E + 005 | 112 | 28–30 | 11.32 | 2.4 | 0.880 |
CAR:RXR·SRC | 0.37 | 0.330 | 45.63 | 45.15 | 729 | 84.0 | 2.05E + 005 | 159 | 37.00 | 17.07 | 3.9 | 0.971 |
CAR(tcp):RXR·SRC | 0.56 | 0.520 | 44.46 | 44.89 | 744 | 100 | 2.15E + 005 | 158 | 36.25 | 17.45 | 3.9 | 0.969 |
CAR(tcp):RXR(9c)·SRC | 1.05 | 0.930 | 55.00 | 54.41 | 1031 | 155 | 3.17E + 005 | 197 | 43.00 | 18.66 | 3.9 | 0.872 |