Skip to main content
. 2014 Jul 22;289(36):24779–24791. doi: 10.1074/jbc.M113.515783

FIGURE 6.

FIGURE 6.

AHA probably does not exert its inflammatory effect through CD44. THP-1 macrophages are treated for 24 h with AHA with and without the CD44 agonist, HA (1500–1800 kDa from S. equi), or antibodies to CD44, and then the media were assayed for cytokines using ELISA, and a measure of the attached cell numbers was determined using the XTT/PMS cell proliferation assay. The data are the means ± S.D. (A, THP-1 secretion of IL-1β in the presence of increasing concentrations of AHA in the presence and absence of HA.THP-1 are incubated with increasing concentrations of AHA in the presence of 50 μg/ml HA (gray bars), 5 μg/ml HA (white bars), or no HA (black bars). B, THP-1 secretion of IL-1β in the presence and absence of AHA and CD44 antibodies (Ab1 and Ab2). Control is THP-1 that is not treated with AHA or antibody to CD44. AHA is used at either 50 or 500 μg/ml and antibody at 5 μg/ml.