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. 2014 Jul 17;289(36):24821–24831. doi: 10.1074/jbc.M114.576546

FIGURE 1.

FIGURE 1.

Antimicrobials accumulate in oyster gills 24 h after infection or injury. Reversed-phase HPLC was performed on gill extracts from non-injected oysters (black line), SSW-injected oysters (green line), and V. tasmaniensis LGP32-injected (LGP32-injected) oysters (purple line) using a 0–80% acetonitrile gradient (dotted line) developed over 90 min, on a UP5ODB-25QS column. Absorbance at 225 nm (continuous lines) showed an increase in intensity for the fractions eluted at 36% of acetonitrile in LGP32-injected (LGP32–36) and SSW-injected oysters (SSW36) and for the fraction eluted at 37% of acetonitrile in LGP32-injected oysters (LGP32–37). Purple and green oblongs show antimicrobial fractions in LGP32-injected and SSW-injected oysters, respectively. LGP32–36, LGP32–37, and SSW36 were the only fractions showing antimicrobial activity against S. aureus SG511. The molecules found by LC-MS/MS in active fractions are displayed with arrows.