FIGURE 3.

The nuclear export inhibitor LepB inhibits Sost expression. A, regulation of HDAC5-cytoplasmic shuttling by LepB, PMA, and forskolin. UMR106 cells were preincubated for 30 min with 40 nm LepB, 100 nm PMA, 10 μm forskolin or solvent control (−) and then stimulated with 100 nm PTH or solvent control for 1 h followed by HCA of HDAC5 nucleocytoplasmic distribution. Shown are mean ± S.E. of three independent experiments normalized to control. B, representative image of PMA-induced HDAC5-GFP nuclear export. Time (C)- and dose (D)-dependent inhibition of Sost expression by LepB. UMR106 cells were incubated with LepB as indicated in the figure and subsequently Sost expression was analyzed by qPCR. Sost expression levels were normalized to β2-microglobulin expression. Shown are relative Sost expression level mean ± S.E. of two independent experiments.