FIGURE 1.

Dose-dependent requirements for phosphatidylserine (left panel) and substrate (right panel) during aPKC assay. Where indicated, mice were injected intraperitoneally with a maximally effective dose of insulin (1 unit/kg body weight) or saline vehicle 15 min before killing. aPKC was immunoprecipitated from liver lysates and assayed with increasing amounts of phosphatidylserine and a preferred aPKC substrate, i.e. the serine analog of the PKC-ϵ pseudosubstrate, along with other standard components of the aPKC assay (see “Experimental Procedures”). Data are mean of 2–4 replicates.