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Effects of PIP3 and PIP2 on activity and phosphorylation of aPKC. aPKC was immunoprecipitated from lysates of livers harvested from basal (i.e. not insulin-stimulated) mice, and assayed in the presence of increasing concentrations of PIP3 (white columns) or PIP2 (black columns). Data are mean ± S.E. of triplicate determinations. Also shown are representative immunoblots of alterations in autophosphorylation (phospho-thr-563/560-PKC-λ/ζ).
