FIGURE 6.

Activation of mouse liver aPKC by treatment with insulin in vivo and/or PIP₃ and ceramide in vitro (a) and by PIP₃in vitro (b). a, where indicated, mice were treated with vehicle or insulin (1 unit/kg body weight) intraperitoneally 15 min before killing. Liver lysates were obtained, and aPKC was collected by immunoprecipitation and incubated with the indicated concentrations of ceramide and PIP₃ and other components of the aPKC assay. b, aPKCs were immunoprecipitated from liver lysates of basal/untreated mice and incubated with the indicated concentrations of PIP₃ and other components of the aPKC assay. After incubation, reaction mixtures were subjected to Western blot analysis for the indicated phosphoproteins and proteins. Representative blots are shown.