FIGURE 12.

siNLRP3 effect on albumin-induced mitochondrial dysfunction in mPTCs. siNLRP3 inhibited albumin-induced mitochondrial dysfunction in mPTCs. Cells were transfected with siNlrp3 for 48 h and then incubated with albumin (10 mg/ml) for 24 h. A and B, ROS production. A, representative images of mPTCs stained with dichlorodihydrofluorescein diacetate. B, quantitation of 2′,7′-dichlorofluorescein fluorescence by flow cytometry. C and D, MMP. C, quantitation of JC-1 fluorescence by flow cytometry. D, representative images JC-1 staining. E, the mtDNA copy number. F, ATP content. The values represent the means ± S.D. (n = 6). *, p < 0.01 versus siNlrp3 group; #, p < 0.01 versus albumin group. Cntl, control.